RRC ID 63454
著者 Sugawara M, Miyoshi H, Miura T, Tanaka H, Tsubota KI, Liu H.
タイトル Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning.
ジャーナル Biomed Res Int
Abstract To understand the mechanism regulating the spontaneous change in polarity that leads to cell turning, we quantitatively analyzed the dynamics of focal adhesions (FAs) coupling with the self-assembling actin cytoskeletal structure in Swiss 3T3 fibroblasts. Fluorescent images were acquired from cells expressing GFP-actin and RFP-zyxin by laser confocal microscopy. On the basis of the maximum area, duration, and relocation distance of FAs extracted from the RFP-zyxin images, the cells could be divided into 3 regions: the front region, intermediate lateral region, and rear region. In the intermediate lateral region, FAs appeared close to the leading edge and were stabilized gradually as its area increased. Simultaneously, bundled actin stress fibers (SFs) were observed vertically from the positions of these FAs, and they connected to the other SFs parallel to the leading edge. Finally, these connecting SFs fused to form a single SF with matured FAs at both ends. This change in SF organization with cell retraction in the first cycle of migration followed by a newly formed protrusion in the next cycle is assumed to lead to cell turning in migrating Swiss 3T3 fibroblasts.
巻・号 2016
ページ 5749749
公開日 2016-1-1
DOI 10.1155/2016/5749749
PMID 28119928
PMC PMC5227335
MeSH Actins / metabolism* Animals Cell Movement / physiology* Cells, Cultured Cytoskeleton / metabolism Fibroblasts / metabolism* Fibroblasts / physiology* Focal Adhesions / metabolism* Focal Adhesions / physiology* Green Fluorescent Proteins / metabolism Mice Microscopy, Fluorescence / methods NIH 3T3 Cells Stress Fibers / metabolism*
IF 2.276
リソース情報
ヒト・動物細胞 3T3 Swiss Albino(RCB1642)