論文 - 詳細
| RRC ID | 63476 |
|---|---|
| 著者 | Nakayama T, Saitoh N, Morotomi-Yano K, Yano K, Nakao M, Saitoh H. |
| タイトル | Nuclear extrusion precedes discharge of genomic DNA fibers during tunicamycin-induced neutrophil extracellular trap-osis (NETosis)-like cell death in cultured human leukemia cells. |
| ジャーナル | Cell Biol Int |
| Abstract |
We previously reported that the nucleoside antibiotic tunicamycin (TN), a protein glycosylation inhibitor triggering unfolded protein response (UPR), induced neutrophil extracellular trap-osis (NETosis)-like cellular suicide and, thus, discharged genomic DNA fibers to extracellular spaces in a range of human myeloid cell lines under serum-free conditions. In this study, we further evaluated the effect of TN on human promyelocytic leukemia HL-60 cells using time-lapse microscopy. Our assay revealed a previously unappreciated early event induced by TN-exposure, in which, at 30-60 min after TN addition, the cells extruded their nuclei into the extracellular space, followed by discharge of DNA fibers to form NET-like structures. Intriguingly, neither nuclear extrusion nor DNA discharge was observed when cells were exposed to inducers of UPR, such as brefeldin A, thapsigargin, or dithiothreitol. Our findings revealed novel nuclear dynamics during TN-induced NETosis-like cellular suicide in HL-60 cells and suggested that the toxicological effect of TN on nuclear extrusion and DNA discharge was not a simple UPR. |
| 巻・号 | 40(5) |
| ページ | 597-602 |
| 公開日 | 2016-5-1 |
| DOI | 10.1002/cbin.10594 |
| PMID | 26888435 |
| MeSH | Anti-Bacterial Agents / pharmacology Apoptosis / drug effects Brefeldin A / pharmacology Cell Death / drug effects Cell Nucleus / metabolism Cells, Cultured DNA, Neoplasm / metabolism Extracellular Traps / metabolism* Glycosylation HL-60 Cells Humans Leukemia / drug therapy* Leukemia / genetics Leukemia / metabolism Leukemia / pathology Neutrophils / metabolism Thapsigargin / pharmacology Tunicamycin / pharmacology* Unfolded Protein Response / drug effects |
| IF | 2.571 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |