RRC ID 63483
著者 Nakatani M, Ito J, Koyama R, Iijima M, Yoshimoto N, Niimi T, Kuroda S, Maturana AD.
タイトル Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2.
ジャーナル Biochem Biophys Res Commun
Abstract Enigma Homolog 1 (ENH1) is a scaffold protein for signaling proteins and transcription factors. Previously, we reported that ENH1 overexpression promotes the differentiation of C2C12 myoblasts. However, the molecular mechanism underlying the role of ENH1 in the C2C12 cells differentiation remains elusive. ENH1 was shown to inhibit the proliferation of neuroblastoma cells by sequestering Inhibitor of DNA binding protein 2 (Id2) in the cytosol. Id2 is a repressor of basic Helix-Loop-Helix transcription factors activity and prevents myogenesis. Here, we found that ENH1 overcome the Id2 repression of C2C12 cells myogenic differentiation and that ENH1 overexpression promotes mice satellite cells activation, the first step toward myogenic differentiation. In addition, we show that ENH1 interacted with Id2 in C2C12 cells and mice satellite cells. Collectively, our results suggest that ENH1 plays an important role in the activation of myogenesis through the repression of Id2 activity.
巻・号 474(2)
ページ 413-420
公開日 2016-5-27
DOI 10.1016/j.bbrc.2016.04.119
PII S0006-291X(16)30633-7
PMID 27114303
MeSH Adaptor Proteins, Signal Transducing / metabolism* Animals Cell Differentiation / physiology Cell Line Inhibitor of Differentiation Protein 1 / metabolism* Mice Microfilament Proteins / metabolism* Muscle Development / physiology* Myoblasts / cytology* Myoblasts / metabolism* Nuclear Matrix-Associated Proteins / metabolism*
IF 2.985
リソース情報
ヒト・動物細胞 C2C12(RCB0987)