Reference - Detail
| RRC ID | 6349 |
|---|---|
| Author | Siripattarapravat K, Pinmee B, Venta PJ, Chang CC, Cibelli JB. |
| Title | Somatic cell nuclear transfer in zebrafish. |
| Journal | Nat Methods |
| Abstract |
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones. |
| Volume | 6(10) |
| Pages | 733-5 |
| Published | 2009-10-1 |
| DOI | 10.1038/nmeth.1369 |
| PII | nmeth.1369 |
| PMID | 19718031 |
| MeSH | Animals Genetic Engineering / methods* Hybrid Cells / transplantation* Nuclear Transfer Techniques Zebrafish / anatomy & histology* Zebrafish / genetics* |
| IF | 30.822 |
| Times Cited | 26 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Resource | |
| Zebrafish | HGn62A HGn28A HGn8E |