Reference - Detail
| RRC ID | 63510 |
|---|---|
| Author | Kaneko S. |
| Title | In Vitro Generation of Antigen-Specific T Cells from Induced Pluripotent Stem Cells of Antigen-Specific T Cell Origin. |
| Journal | Methods Mol Biol |
| Abstract |
Induced pluripotent stem (iPS) cells derived from T lymphocyte (T-iPS cells) preserve the T cell receptor (TCR) α and β gene rearrangements identical to the original T cell clone. Re-differentiated CD8 single positive αβ T cells from the T-iPS cells exhibited antigen-specific cytotoxicity, improved proliferative response, and elongation of telomere indicating rejuvenation of antigen specific T cell immunity in vitro. To regenerate antigen specific cytotoxic T lymphocytes (CTL), first, we have optimized a method for reprogramming-resistant CD8 T cell clones into T-iPS cells by using sendaiviral vectors. Second, we have optimized stepwise differentiation methods for inducing hematopoietic progenitor cells, T cell progenitors, and functionally matured CD8 single positive CTL. These protocols provide useful in vitro tools and models both for research of antigen-specific T cell immunotherapy and for research of normal and pathological thymopoiesis. |
| Volume | 1393 |
| Pages | 67-73 |
| Published | 2016-1-1 |
| DOI | 10.1007/978-1-4939-3338-9_6 |
| PMID | 27033216 |
| MeSH | Animals CD8-Positive T-Lymphocytes / metabolism* Cell Differentiation Cells, Cultured Cellular Reprogramming* Cytotoxicity, Immunologic Humans Induced Pluripotent Stem Cells / physiology* Mice Receptors, Antigen, T-Cell, alpha-beta / metabolism T-Lymphocytes, Cytotoxic / metabolism* |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) OP9/DL1 |