RRC ID 63587
Author Kikuchi M, Nishimura T, Ishishita S, Matsuda Y, Tanaka M.
Title foxl3, a sexual switch in germ cells, initiates two independent molecular pathways for commitment to oogenesis in medaka.
Journal Proc Natl Acad Sci U S A
Abstract Germ cells have the ability to differentiate into eggs and sperm and must determine their sexual fate. In vertebrates, the mechanism of commitment to oogenesis following the sexual fate decision in germ cells remains unknown. Forkhead-box protein L3 (foxl3) is a switch gene involved in the germline sexual fate decision in the teleost fish medaka (Oryzias latipes). Here, we show that foxl3 organizes two independent pathways of oogenesis regulated by REC8 meiotic recombination protein a (rec8a), a cohesin component, and F-box protein (FBP) 47 (fbxo47), a subunit of E3 ubiquitin ligase. In mutants of either gene, germ cells failed to undergo oogenesis but developed normally into sperm in testes. Disruption of rec8a resulted in arrest at a meiotic pachytenelike stage specifically in females, revealing a sexual difference in meiotic progression. Analyses of fbxo47 mutants showed that this gene regulates transcription factors that facilitate folliculogenesis: LIM homeobox 8 (lhx8b), factor in the germline α (figla), and newborn ovary homeobox (nobox). Interestingly, we found that the fbxo47 pathway ensures that germ cells do not deviate from an oogenic pathway until they reach diplotene stage. The mutant phenotypes together with the timing of their expression imply that germline feminization is established during early meiotic prophase I.
Volume 117(22)
Pages 12174-12181
Published 2020-6-2
DOI 10.1073/pnas.1918556117
PII 1918556117
PMID 32409601
PMC PMC7275758
MeSH Animals Female Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation, Developmental Germ Cells / cytology* Germ Cells / physiology Gonads / embryology* Gonads / metabolism Male Meiosis Oogenesis* Oryzias / growth & development* Ovarian Follicle / cytology Ovarian Follicle / physiology* Sex Determination Processes Spermatogenesis Spermatozoa / cytology Spermatozoa / physiology*
IF 9.412
Medaka OK-Cab (MT830) cDNA clone (ola1-212j01, olte61d07, olova4m02)