RRC ID 63593
Author Liu F, Zhu C, Gao P, Zheng S, Li C.
Title Ubiquitin-conjugating enzyme E2T regulates cell proliferation and migration in cholangiocarcinoma.
Journal Anticancer Drugs
Abstract Ubiquitin-conjugating enzyme E2T (UBE2T) is overexpressed in several human cancer cells, but a role in cholangiocarcinoma (CAA) progression has not been investigated. We analyzed the expression of UBE2T in CAA tissues. Then, we generated UBE2T deregulation models in which it was overexpressed or silenced, and examined the effects on CAA malignant progression by flow cytometry, western blot, MTT assay, wound healing assay and transwell assay. We report the involvement of UBE2T in CAA malignant progression. UBE2T was found to be highly expressed in human CAA cells both in vitro and in vivo. Overexpression of UBE2T significantly enhanced epithelial-to-mesenchymal transition, proliferation, migration and invasion of CAA cells in vitro, while silencing UBE2T had opposing effects. Furthermore, UBE2T appears to exert its effects via the mammalian target of rapamycin (mTOR) pathway as the cellular effects caused by UBE2T overexpression are inhibited by the mTOR inhibitor rapamycin. Our findings suggest that UBE2T may have potential as a new therapeutic target for the prevention or treatment of CAA.
Volume 31(8)
Pages 836-846
Published 2020-9-1
DOI 10.1097/CAD.0000000000000955
PII 00001813-202009000-00009
PMID 32796405
MeSH Apoptosis Bile Duct Neoplasms / genetics Bile Duct Neoplasms / metabolism Bile Duct Neoplasms / pathology* Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Cell Cycle Cell Movement* Cell Proliferation* Cholangiocarcinoma / genetics Cholangiocarcinoma / metabolism Cholangiocarcinoma / pathology* Disease Progression Epithelial-Mesenchymal Transition Female Gene Expression Regulation, Neoplastic* Humans Male Neoplasm Invasiveness Prognosis Tumor Cells, Cultured Ubiquitin-Conjugating Enzymes / genetics Ubiquitin-Conjugating Enzymes / metabolism*
IF 2.26
Human and Animal Cells HuCCT1(RCB1960)