| RRC ID |
63598
|
| 著者 |
Hirata H, Kuwatani M, Nakajima K, Kodama Y, Yoshikawa Y, Ogawa M, Sakamoto N.
|
| タイトル |
Near-infrared photoimmunotherapy (NIR-PIT) on cholangiocarcinoma using a novel catheter device with light emitting diodes.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
Near-infrared photoimmunotherapy (NIR-PIT) is a novel therapy for cancers that uses NIR light and antibody-photosensitizer (IR700) conjugates. However, it is difficult to deliver NIR light into the bile duct for cholangiocarcinoma (CCA) from the conventional extracorporeal apparatus. Thus, in this study, we developed a dedicated catheter with light emitting diodes (LEDs) that supersedes conventional external irradiation devices; we investigated the therapeutic effect of NIR-PIT for CCA using the novel catheter. The new catheter was designed to be placed in the bile duct and a temperature sensor was attached to the tip of the catheter to avoid thermal burn. An anti-epidermal growth factor receptor (EGFR) antibody, Panitumumab-IR700 conjugate or anti-human epidermal growth factor receptor type 2 (HER2) antibody, Trastuzumab-IR700 conjugate, was used with EGFR- or HER2-expressing cell lines, respectively. The in vitro efficacy of NIR-PIT was confirmed in cultured cells; the capability of the new catheter for NIR-PIT was then tested in a mouse tumor model. NIR-PIT via the developed catheter treated CCA xenografts in mice. NIR-PIT had an effect in Panitumumab-IR700 conjugate- and Trastuzumab-IR700 conjugate-treated CCA cells that depended on the receptor expression level. Tumor growth was significantly suppressed in mice treated with NIR-PIT using the novel catheter compared with controls (P < .01). NIR-PIT was an effective treatment for EGFR- and HER2-expressing CCA cells, and the novel catheter with mounted LEDs was useful for NIR-PIT of CCA.
|
| 巻・号 |
112(2)
|
| ページ |
828-838
|
| 公開日 |
2021-2-1
|
| DOI |
10.1111/cas.14780
|
| PMID |
33345417
|
| PMC |
PMC7894014
|
| MeSH |
Animals
Bile Duct Neoplasms / therapy*
Catheters
Cell Line, Tumor
Cholangiocarcinoma / therapy*
Female
Humans
Immunotherapy / instrumentation*
Immunotherapy / methods
Infrared Rays / therapeutic use
Low-Level Light Therapy / instrumentation*
Low-Level Light Therapy / methods
Mice
Mice, Inbred BALB C
Mice, Nude
Panitumumab / pharmacology
Photosensitizing Agents / pharmacology
Trastuzumab / pharmacology
Xenograft Model Antitumor Assays
|
| IF |
4.966
|
| リソース情報 |
| ヒト・動物細胞 |
HuCCT1(RCB1960)
YSCCC(RCB1549)
TFK-1(RCB2537) |
