論文 - 詳細
| RRC ID | 6362 |
|---|---|
| 著者 | Gillan V, Maitland K, McCormack G, Him NA, Devaney E. |
| タイトル | Functional genomics of hsp-90 in parasitic and free-living nematodes. |
| ジャーナル | Int J Parasitol |
| Abstract |
Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C. elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans. |
| 巻・号 | 39(10) |
| ページ | 1071-81 |
| 公開日 | 2009-8-1 |
| DOI | 10.1016/j.ijpara.2009.02.024 |
| PII | S0020-7519(09)00167-2 |
| PMID | 19401205 |
| PMC | PMC2845815 |
| MeSH | Animals Brugia pahangi / genetics Caenorhabditis elegans / genetics Gene Expression Regulation / genetics* Genes, Helminth / genetics* HSP90 Heat-Shock Proteins / genetics* HSP90 Heat-Shock Proteins / metabolism Haemonchus / genetics Molecular Sequence Data Mutation Nematoda / genetics* Nematoda / parasitology Sequence Analysis, DNA |
| IF | 3.53 |
| 引用数 | 30 |
| WOS 分野 | PARASITOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3133 |