| RRC ID |
6362
|
| 著者 |
Gillan V, Maitland K, McCormack G, Him NA, Devaney E.
|
| タイトル |
Functional genomics of hsp-90 in parasitic and free-living nematodes.
|
| ジャーナル |
Int J Parasitol
|
| Abstract |
Heat shock protein 90 (Hsp-90) is a highly conserved essential protein in eukaryotes. Here we describe the molecular characterisation of hsp-90 from three nematodes, the free-living Caenorhabditis elegans (Ce) and the parasitic worms Brugia pahangi (Bp) and Haemonchus contortus (Hc). These molecules were functionally characterised by rescue of a Ce-daf-21 (hsp-90) null mutant. Our results show a gradient of rescue: the C. elegans endogenous gene provided full rescue of the daf-21 mutant, while Hc-hsp-90 provided partial rescue. In contrast, no rescue could be obtained using a variety of Bp-hsp-90 constructs, despite the fact that Bp-hsp-90 was transcribed and translated in the mutant worms. daf-21 RNA interference (RNAi) experiments were carried out to determine whether knock-down of the endogenous daf-21 mRNA in N2 worms could be complemented by expression of either parasite gene. However neither parasite gene could rescue the daf-21 (RNAi) phenotypes. These results indicate that factors other than the level of sequence identity are important for determining whether parasite genes can functionally complement in C. elegans.
|
| 巻・号 |
39(10)
|
| ページ |
1071-81
|
| 公開日 |
2009-8-1
|
| DOI |
10.1016/j.ijpara.2009.02.024
|
| PII |
S0020-7519(09)00167-2
|
| PMID |
19401205
|
| PMC |
PMC2845815
|
| MeSH |
Animals
Brugia pahangi / genetics
Caenorhabditis elegans / genetics
Gene Expression Regulation / genetics*
Genes, Helminth / genetics*
HSP90 Heat-Shock Proteins / genetics*
HSP90 Heat-Shock Proteins / metabolism
Haemonchus / genetics
Molecular Sequence Data
Mutation
Nematoda / genetics*
Nematoda / parasitology
Sequence Analysis, DNA
|
| IF |
3.53
|
| 引用数 |
30
|
|
WOS 分野
|
PARASITOLOGY
|
| リソース情報 |
| 線虫 |
tm3133 |
