| RRC ID |
63647
|
| 著者 |
Raju R, Oshima M, Inoue M, Morita T, Huijiao Y, Waskitho A, Baba O, Inoue M, Matsuka Y.
|
| タイトル |
Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
|
| 巻・号 |
10(1)
|
| ページ |
1656
|
| 公開日 |
2020-2-3
|
| DOI |
10.1038/s41598-020-58222-0
|
| PII |
10.1038/s41598-020-58222-0
|
| PMID |
32015383
|
| PMC |
PMC6997427
|
| MeSH |
3T3 Cells
Animals
Cells, Cultured
Dental Cementum / cytology
Dental Cementum / physiology
Dental Cementum / transplantation
Female
Guided Tissue Regeneration, Periodontal / methods
Imaging, Three-Dimensional
Male
Mice
Mice, Inbred C57BL
Mice, SCID
Osteoblasts / cytology
Osteoblasts / physiology
Osteoblasts / transplantation
Periodontal Ligament / cytology
Periodontal Ligament / physiology
Periodontal Ligament / transplantation
Periodontium / anatomy & histology
Periodontium / cytology
Periodontium / physiology*
Rats
Rats, Sprague-Dawley
Regeneration / physiology*
Tissue Engineering / methods
X-Ray Microtomography
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
