RRC ID 63656
著者 Mizutani Y, Omagari D, Hayatsu M, Nameta M, Komiyama K, Mikami Y, Ushiki T.
タイトル SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
ジャーナル Cell Adh Migr
Abstract To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
巻・号 14(1)
ページ 195-203
公開日 2020-12-1
DOI 10.1080/19336918.2020.1829264
PMID 33016205
PMC PMC7553583
MeSH Cell Line, Tumor Cell Movement* Cell Proliferation Desmosomes / metabolism* Desmosomes / ultrastructure Galectin 4 / genetics Galectin 4 / metabolism* Humans Pseudopodia / metabolism* Pseudopodia / ultrastructure RNA, Messenger / genetics RNA, Messenger / metabolism Secretory Leukocyte Peptidase Inhibitor / metabolism*
IF 3.694
リソース情報
ヒト・動物細胞 Ca9-22(RCB1976)