論文 - 詳細
| RRC ID | 63676 |
|---|---|
| 著者 | Takayama I, Kondo N, Kalies S, Heisterkamp A, Terakawa M. |
| タイトル | Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes. |
| ジャーナル | J Biophotonics |
| Abstract |
Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation. |
| 巻・号 | 13(7) |
| ページ | e202000037 |
| 公開日 | 2020-7-1 |
| DOI | 10.1002/jbio.202000037 |
| PMID | 32250039 |
| MeSH | Cell Proliferation Lasers Myoblasts* Polymers Tissue Engineering* |
| IF | 3.032 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ChR2-C2C12(RCB4693) |