論文 - 詳細
| RRC ID | 6369 |
|---|---|
| 著者 | Hammell CM, Lubin I, Boag PR, Blackwell TK, Ambros V. |
| タイトル | nhl-2 Modulates microRNA activity in Caenorhabditis elegans. |
| ジャーナル | Cell |
| Abstract |
TRIM-NHL proteins represent a large class of metazoan proteins implicated in development and disease. We demonstrate that a C. elegans TRIM-NHL protein, NHL-2, functions as a cofactor for the microRNA-induced silencing complex (miRISC) and thereby enhances the posttranscriptional repression of several genetically verified microRNA targets, including hbl-1 and let-60/Ras (by the let-7 family of microRNAs) and cog-1 (by the lsy-6 microRNA). NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core miRISC components ALG-1/2 and AIN-1. nhl-2 and cgh-1 mutations compromise the repression of microRNA targets in vivo but do not affect microRNA biogenesis, indicating a role for an NHL-2:CGH-1 complex in the effector phase of miRISC activity. We propose that the NHL-2:CGH-1 complex functions in association with mature miRISC to modulate the efficacy of microRNA:target interactions in response to physiological and developmental signals, thereby ensuring the robustness of genetic regulatory pathways regulated by microRNAs. |
| 巻・号 | 136(5) |
| ページ | 926-38 |
| 公開日 | 2009-3-6 |
| DOI | 10.1016/j.cell.2009.01.053 |
| PII | S0092-8674(09)00150-0 |
| PMID | 19269369 |
| PMC | PMC2670343 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cytoplasmic Granules / metabolism Homeodomain Proteins / metabolism MicroRNAs / metabolism* RNA Nucleotidyltransferases / genetics RNA Nucleotidyltransferases / metabolism RNA, Helminth / metabolism* RNA-Induced Silencing Complex / metabolism |
| IF | 38.637 |
| 引用数 | 114 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm369 tm2516 |