| RRC ID |
63696
|
| 著者 |
Wu H, He Y, Wu H, Zhou M, Xu Z, Xiong R, Yan F, Liu H.
|
| タイトル |
Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes.
|
| ジャーナル |
Theranostics
|
| Abstract |
Rheumatoid arthritis (RA), a common inflammatory disorder of the joints characterized by synovitis and pannus formation, often results in irreversible joint erosion and disability. Methotrexate (MTX) is the first-line drug against RA, but the therapeutic effects are sub-optimal due to its poor retention at the target sites and systemic side effects. Multifunctional nanoparticles are highly promising agents for minimally invasive, traceable and effective targeted therapy. Methods: This study developed iRGD peptide-functionalized echogenic liposomes (iELPs) which encapsulates MTX and indocyanine green (ICG) fluorescent probe through the thin film-hydration method. Results: The resulting iELPs showed high affinity for endothelial cells overexpressing αvβ3 integrin, favorable acoustic response and fluorescence tracking properties. Also, near-infrared (NIR) fluorescence imaging of iELPs and ultrasound-triggered drug release of MTX were proved in a mouse RA model, greatly improving the therapeutic efficacy and reducing MTX side effects. Histological assessment of the articular tissues further revealed significantly lower inflammatory cell infiltration and angiogenesis in the iELPs-treated and sonicated mice. Conclusion: Our study provides a promising nanoplatform for integrating ultrasound-controlled drug release and NIR fluorescence imaging for RA treatment.
|
| 巻・号 |
10(22)
|
| ページ |
10092-10105
|
| 公開日 |
2020-1-1
|
| DOI |
10.7150/thno.44865
|
| PII |
thnov10p10092
|
| PMID |
32929336
|
| PMC |
PMC7481417
|
| MeSH |
Animals
Arthritis, Rheumatoid / drug therapy*
Cell Line
Endothelial Cells / drug effects
Endothelial Cells / metabolism
Fluorescent Dyes / administration & dosage*
Human Umbilical Vein Endothelial Cells
Humans
Indocyanine Green / metabolism
Integrin alphaVbeta3 / metabolism
Liposomes / metabolism*
Male
Methotrexate / pharmacology*
Mice
Oligopeptides / pharmacology*
Optical Imaging / methods*
RAW 264.7 Cells
Spectroscopy, Near-Infrared / methods
Swine
Ultrasonic Therapy / methods*
Ultrasonic Waves
|
| IF |
8.579
|
| リソース情報 |
| ヒト・動物細胞 |
MH7A(RCB1512) |
