論文 - 詳細
| RRC ID | 63700 |
|---|---|
| 著者 | Nomi K, Hayashi R, Ishikawa Y, Kobayashi Y, Katayama T, Quantock AJ, Nishida K. |
| タイトル | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs. |
| ジャーナル | Cell Rep |
| Abstract |
The conjunctival epithelium, which covers the sclera (the white of the eye) and lines the inside of the eyelids, is essential for mucin secretion and the establishment of a healthy tear film. Here, we describe human conjunctival development in a self-formed ectodermal autonomous multi-zone (SEAM) of cells that were derived from human-induced pluripotent stem cells (hiPSCs) and mimic whole-eye development. Our data indicate that epidermal growth factor (EGF) drives the generation of cells with a conjunctival epithelial lineage. We also show that individual conjunctival cells can be sorted and reconstituted by cultivation into a functional conjunctival epithelium that includes mucin-producing goblet cells. Keratinocyte growth factor (KGF), moreover, is necessary for the maturation of hiPSC-derived conjunctival epithelium-particularly the goblet cells-indicating key complementary roles of EGF and KGF in directing the differentiation and maturation, respectively, of the human conjunctival epithelium. |
| 巻・号 | 34(5) |
| ページ | 108715 |
| 公開日 | 2021-2-2 |
| DOI | 10.1016/j.celrep.2021.108715 |
| PII | S2211-1247(21)00028-0 |
| PMID | 33535050 |
| MeSH | Cell Differentiation Conjunctiva / metabolism* Goblet Cells / metabolism* Humans Induced Pluripotent Stem Cells / metabolism* |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |