論文 - 詳細
| RRC ID | 63741 |
|---|---|
| 著者 | Herr P, Boström J, Rullman E, Rudd SG, Vesterlund M, Lehtiö J, Helleday T, Maddalo G, Altun M. |
| タイトル | Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics. |
| ジャーナル | Mol Cell Proteomics |
| Abstract |
The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics. |
| 巻・号 | 19(4) |
| ページ | 608-623 |
| 公開日 | 2020-4-1 |
| DOI | 10.1074/mcp.RA120.001938 |
| PII | S1535-9476(20)35020-9 |
| PMID | 32051232 |
| PMC | PMC7124475 |
| MeSH | Cell Cycle / genetics* Cell Nucleus / metabolism Gene Expression Profiling* HeLa Cells Humans Neoplasm Proteins / genetics* Neoplasm Proteins / metabolism Phosphorylation Protein Transport Proteome / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Subcellular Fractions / metabolism Transcriptome / genetics |
| IF | 4.87 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |