| RRC ID |
63743
|
| Author |
Miyazaki T, Hanamatsu H, Xu L, Onodera T, Furukawa JI, Homan K, Baba R, Kawasaki T, Iwasaki N.
|
| Title |
Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique.
|
| Journal |
Int J Mol Sci
|
| Abstract |
Cartilage damage may eventually lead to osteoarthritis because it is difficult to repair. Human-induced pluripotent stem cell (iPSC)-derived chondrocytes may potentially be used to treat cartilage damage, but the tumorigenicity of iPSCs is a major concern for their application in regenerative medicine. Many glycoconjugates serve as stem cell markers, and glycosphingolipids (GSLs) including H type 1 antigen (Fucα1-2Galβ1-3GlcNAc) have been expressed on the surface of iPSCs. The purpose of the present study was to investigate whether GSL-glycome analysis is useful for quality control of residual iPSCs in chondrocytes. We performed GSL-glycome analysis of undifferentiated iPSCs in chondrocytes by combining glycoblotting and aminolysis-sialic acid linkage-specific alkylamidation (SALSA) method, enabling the detection of small quantities of iPSC-specific GSL-glycans from 5 × 104 cells. Furthermore, we estimated the residual amount of iPSCs using R-17F antibody, which possesses cytotoxic activity toward iPSCs that is dependent on the Lacto-N-fucopentaose I (LNFP I) of GSL. Moreover, we could detect a small number of LNFP I during mesenchymal stem cells (MSCs) differentiation from iPSCs. This is the first demonstration that GSL-glycome analysis is useful for detecting undifferentiated iPSCs, and can thereby support safe regenerative medicine.
|
| Volume |
21(1)
|
| Published |
2019-12-28
|
| DOI |
10.3390/ijms21010231
|
| PII |
ijms21010231
|
| PMID |
31905707
|
| PMC |
PMC6982033
|
| MeSH |
Cell Differentiation*
Cell Line
Cells, Cultured
Chondrocytes / cytology
Chondrocytes / metabolism*
Glycomics / methods
Glycosphingolipids / metabolism*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Oligosaccharides / metabolism
|
| IF |
4.556
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063)
606A1(HPS0328) |
