RRC ID 63752
Author Tao F, Wang F, Zhang W, Hao Y.
Title MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells.
Journal Eur J Histochem
Abstract Considerable evidence has verified that microRNAs (miRNAs) play important roles in various cellular processes including differentiation. However, the regulatory roles of miRNAs involved in the differentiation of induced pluripotent stem cells (iPSC) into lung epithelial cells are still unknown. In this study, we first evaluated the current protocols to differentiate iPSC into alveolar epithelial type II (AEC II) cells, but the efficiency is low. We next identified that miR-22 can efficiently enhance the differentiation of iPSC into AEC II cells under the stimulation of proper growth factors and growing on appropriate matrix. Moreover, the AEC II cells generated from iPSC with miR-22 overexpression can proliferate and secrete lung surfactant. Here, we discovered a previously unknown interaction between miR-22 and iPSC differentiation but also provide a potential target for the effective derivation of AEC II from iPSCs for cell-based therapy.
Volume 64(s2)
Published 2020-10-1
DOI 10.4081/ejh.2020.3170
PMID 33334090
PMC PMC7542669
MeSH Alveolar Epithelial Cells / metabolism* Animals Cell Differentiation / physiology* HEK293 Cells Humans Induced Pluripotent Stem Cells / metabolism* Male Mice, Inbred C57BL MicroRNAs / metabolism* Pulmonary Surfactant-Associated Proteins / metabolism
IF 2.172
Human and Animal Cells iPS-MEF-Ng-492B-4(APS0004)