RRC ID 63788
著者 Quan H, Kim Y, Wu L, Park HC, Yang HC.
タイトル Modulation of Macrophage Polarization by Phospholipids on the Surface of Titanium.
ジャーナル Molecules
Abstract Macrophage polarization has become increasingly important for the improvement of the biocompatibility of biomaterials. In this study, we coated Ti discs with phospholipids (phosphatidylserine/phosphatidylcholine [4:1 mole/mole]) by evaporating the solvent under vacuum, and observed the polarization of RAW 264.7 cells cultured on the discs. The coated discs were hydrated before cell culture was added. The shape of the hydrated phospholipids varied with the concentration of loaded phospholipids: a perforated layer (0.1 mM), tubules and spheres (1 mM), and spheres (10 mM). RAW 264.7 cells exhibited different morphologies, depending on the concentration of phospholipids. On the coated discs, the gene expression and protein release of TGF-β1, VEGF, Arg-1, and TNF-α were downregulated, especially with 10 mM phospholipids. The stimulation of mRNA expression and the protein release of those genes by IL-4 and LPS were also disturbed on the phospholipid-coated discs. In conclusion, the polarization of RAW 264.7 cells was prevented by hydrated phospholipids on Ti discs.
巻・号 25(11)
公開日 2020-6-10
DOI 10.3390/molecules25112700
PII molecules25112700
PMID 32532146
PMC PMC7321141
MeSH Animals Cell Adhesion Cell Proliferation Cells, Cultured Macrophage Activation / drug effects* Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Mice Phospholipids / chemistry Phospholipids / pharmacology* Surface Properties Titanium / chemistry* Transforming Growth Factor beta1 / metabolism
IF 3.267
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)