論文 - 詳細
| RRC ID | 63798 |
|---|---|
| 著者 | Koga T, Sakamoto T, Sakuradani E, Tai A. |
| タイトル | Neurite Outgrowth-Promoting Activity of Compounds in PC12 Cells from Sunflower Seeds. |
| ジャーナル | Molecules |
| Abstract |
In the current super-aging society, the establishment of methods for prevention and treatment of Alzheimer's disease (AD) is an urgent task. One of the causes of AD is thought to be a decrease in the revel of nerve growth factor (NGF) in the brain. Compounds showing NGF-mimicking activity and NGF-enhancing activity have been examined as possible agents for improving symptoms. In the present study, sunflower seed extract was found to have neurite outgrowth-promoting activity, which is an NGF-enhancing activity, in PC12 cells. To investigate neurite outgrowth-promoting compounds from sunflower seed extract, bioassay-guided purification was carried out. The purified active fraction was obtained by liquid-liquid partition followed by some column chromatographies. Proton nuclear magnetic resonance and gas chromatography-mass spectrometry analyses of the purified active fraction indicated that the fraction was a mixture of β-sitosterol, stigmasterol and campesterol, with β-sitosterol being the main component. Neurite outgrowth-promoting activities of β-sitosterol, stigmasterol, campesterol and cholesterol were evaluated in PC12 cells. β-Sitosterol and stigmasterol showed the strongest activity of the four sterol compounds (β-sitosterol ≈ stigmasterol > campesterol > cholesterol), and cholesterol did not show any activity. The results indicated that β-sitosterol was the major component responsible for the neurite outgrowth-promoting activity of sunflower seeds. Results of immunostaining also showed that promotion by β-sitosterol of neurite formation induced by NGF was accompanied by neurofilament expression. β-Sitosterol, which showed NGF-enhancing activity, might be a candidate ingredient in food for prevention of AD. |
| 巻・号 | 25(20) |
| 公開日 | 2020-10-16 |
| DOI | 10.3390/molecules25204748 |
| PII | molecules25204748 |
| PMID | 33081156 |
| PMC | PMC7587564 |
| MeSH | Alzheimer Disease / drug therapy* Alzheimer Disease / genetics Animals Brain / drug effects Cholesterol / analogs & derivatives Cholesterol / pharmacology Gene Expression Regulation / drug effects Helianthus / chemistry* Humans Nerve Growth Factor / genetics Neurites / drug effects Neuronal Outgrowth / drug effects PC12 Cells Phytosterols / pharmacology Plant Extracts / chemistry Plant Extracts / pharmacology* Rats Seeds / chemistry Sitosterols / pharmacology Stigmasterol / pharmacology |
| IF | 3.267 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |