| RRC ID |
63810
|
| 著者 |
Harada M, Nghia VT, Nakao A, Tanigaki R, Fukuoka N, Nishida A, Kataoka T.
|
| タイトル |
Eomesodermin promotes interaction of RelA and NFATc2 with the Ifng promoter and multiple conserved noncoding sequences across the Ifng locus in mouse lymphoma BW5147 cells.
|
| ジャーナル |
Immunol Lett
|
| Abstract |
The T-box transcription factor Eomesodermin (Eomes) regulates the lineage-dependent expression of interferon γ (IFN-γ). We previously showed that Eomes promotes IFN-γ production and interacts with multiple conserved noncoding sequences (CNS) across the Ifng locus in mouse lymphoma BW5147 cells. In the present study, we investigated the transcriptional regulation of IFN-γ by the nuclear factor κB (NF-κB) subunit RelA and nuclear factor of activated T cells c2 (NFATc2, also known as NFAT1) in Eomes-transfected BW5147 cells. Eomes promoted the interaction of RelA and NFATc2 with the Ifng promoter and five CNS, including CNS-22 and CNS+30 upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (IM). The dual NF-κB and STAT3 inhibitor TPCA-1 moderately reduced the PMA- and IM-induced IFN-γ transcription in Eomes-transfected BW5147 cells. TPCA-1 interfered with RelA binding to the Ifng promoter, CNS-22 and CNS+30. Moreover, TPCA-1 reduced the interaction of Eomes or NFATc2 with the Ifng promoter and CNS+30. The present results indicate that Eomes promotes the interaction of RelA and NFATc2 with the Ifng promoter and multiple CNS across the Ifng locus in BW5147 cells.
|
| 巻・号 |
225
|
| ページ |
33-43
|
| 公開日 |
2020-9-1
|
| DOI |
10.1016/j.imlet.2020.06.008
|
| PII |
S0165-2478(20)30333-3
|
| PMID |
32554052
|
| MeSH |
Amides / therapeutic use*
Animals
Cell Line, Tumor
Conserved Sequence / genetics
Gene Expression Regulation, Neoplastic
Genetic Loci / genetics
Interferon-gamma / genetics
Lymphoma / drug therapy
Lymphoma / genetics*
Mice
NFATC Transcription Factors / metabolism*
Promoter Regions, Genetic / genetics
Protein Kinase Inhibitors / pharmacology
STAT3 Transcription Factor / antagonists & inhibitors
T-Box Domain Proteins / metabolism*
Thiophenes / therapeutic use*
Transcription Factor RelA / metabolism*
|
| IF |
3.276
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
