論文 - 詳細
| RRC ID | 63835 |
|---|---|
| 著者 | Sekiya-Aoyama R, Arisaka Y, Yui N. |
| タイトル | Mobility Tuning of Polyrotaxane Surfaces to Stimulate Myocyte Differentiation. |
| ジャーナル | Macromol Biosci |
| Abstract |
Polyrotaxanes, consisting of poly(ethylene glycol) and α-cyclodextrins, are mechanically interlocked supermolecules. The structure allows α-cyclodextrins to move along the polymer, referred to as molecular mobility. Here, polyrotaxane-based triblock copolymers, composed of polyrotaxanes with different degrees of methylation and poly(benzyl methacrylate) at both terminals, are coated on culture surfaces to fabricate dynamic biointerfaces for myocyte differentiation. The molecular mobility increases with the degree of methylation and the contact angle hysteresis of water droplets and air bubbles. When the mouse myoblast cell line C2C12 is cultured on methylated polyrotaxane surfaces, the expression levels of myogenesis-related genes, myogenin (Myog) and myosin heavy chain (Myhc) are altered by the degree of methylation. Polyrotaxane surfaces with intermediate degrees of methylation promote the highest expression levels among all the surfaces. The polyrotaxane surface provides an appropriate environment for myocyte differentiation by accurately adjusting the degrees of methylation. |
| 巻・号 | 20(4) |
| ページ | e1900424 |
| 公開日 | 2020-4-1 |
| DOI | 10.1002/mabi.201900424 |
| PMID | 32058659 |
| MeSH | Air / analysis Animals Biocompatible Materials / chemical synthesis* Biocompatible Materials / pharmacology Biomarkers / metabolism Cell Differentiation / drug effects Cell Line Cyclodextrins / chemical synthesis* Cyclodextrins / pharmacology Gene Expression Methylation Mice Muscle Cells / cytology Muscle Cells / drug effects* Muscle Cells / metabolism Muscle Development / drug effects* Muscle Development / genetics Myoblasts / cytology Myoblasts / drug effects* Myoblasts / metabolism Myogenin / genetics Myogenin / metabolism Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism Poloxamer / chemical synthesis* Poloxamer / pharmacology Polymethacrylic Acids / chemistry* Rotaxanes / chemical synthesis* Rotaxanes / pharmacology Structure-Activity Relationship Water / chemistry |
| IF | 3.416 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |