RRC ID 63838
Author Guo W, Mashimo Y, Kobatake E, Mie M.
Title Construction of DNA-displaying nanoparticles by enzymatic conjugation of DNA and elastin-like polypeptides using a replication initiation protein.
Journal Nanotechnology
Abstract DNA-displaying nanoparticles comprised of conjugates of single-stranded DNA (ssDNA) and elastin-like polypeptide (ELP) were developed. ssDNA was enzymatically conjugated to ELPs via a catalytic domain of Porcine Circovirus type 2 replication initiation protein (pRep) fused to ELPs. Nanoparticles were formed upon heating to temperatures above the phase transition temperature due to the hydrophobicity of ELPs and the hydrophilicity of conjugated ssDNA. We demonstrated the applicability of the resultant nanoparticles as drug carriers with tumor-targeting properties by conjugating a DNA aptamer, which is known to bind to Mucin 1 (MUC1), to ELPs. DNA aptamer-displaying nanoparticles encapsulating the anti-cancer drug paclitaxel were able to bind to cells overexpressing MUC1 and induce cell death.
Volume 31(25)
Pages 255102
Published 2020-4-3
DOI 10.1088/1361-6528/ab8042
PMID 32176872
MeSH Aptamers, Nucleotide / chemistry Cell Survival / drug effects Circovirus / genetics Circovirus / metabolism DNA Replication DNA, Single-Stranded / chemistry* Drug Carriers Elastin / chemistry* Humans Hydrophobic and Hydrophilic Interactions MCF-7 Cells Nanoparticles Paclitaxel / chemistry Paclitaxel / pharmacology* Peptides / chemistry* Viral Proteins / chemistry*
IF 3.551
Human and Animal Cells MCF7(RCB1904)