RRC ID |
6385
|
Author |
Kim K, Sato K, Shibuya M, Zeiger DM, Butcher RA, Ragains JR, Clardy J, Touhara K, Sengupta P.
|
Title |
Two chemoreceptors mediate developmental effects of dauer pheromone in C. elegans.
|
Journal |
Science
|
Abstract |
Intraspecific chemical communication is mediated by signals called pheromones. Caenorhabditis elegans secretes a mixture of small molecules (collectively termed dauer pheromone) that regulates entry into the alternate dauer larval stage and also modulates adult behavior via as yet unknown receptors. Here, we identify two heterotrimeric GTP-binding protein (G protein)-coupled receptors (GPCRs) that mediate dauer formation in response to a subset of dauer pheromone components. The SRBC-64 and SRBC-66 GPCRs are members of the large Caenorhabditis-specific SRBC subfamily and are expressed in the ASK chemosensory neurons, which are required for pheromone-induced dauer formation. Expression of both, but not each receptor alone, confers pheromone-mediated effects on heterologous cells. Identification of dauer pheromone receptors will allow a better understanding of the signaling cascades that transduce the context-dependent effects of ecologically important chemical signals.
|
Volume |
326(5955)
|
Pages |
994-8
|
Published |
2009-11-13
|
DOI |
10.1126/science.1176331
|
PII |
1176331
|
PMID |
19797623
|
PMC |
PMC4448937
|
MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology
Calcium / metabolism
Cell Line
Chemoreceptor Cells / metabolism
Cyclic AMP / metabolism
Cyclic GMP / metabolism
GTP-Binding Protein alpha Subunits, Gi-Go / physiology
Gene Expression Regulation, Developmental
Genes, Helminth
Guanylate Cyclase / antagonists & inhibitors
Guanylate Cyclase / metabolism
Hexoses / chemistry
Hexoses / physiology
Humans
Mutation
Pheromones / physiology*
Receptors, G-Protein-Coupled
Reproduction
Signal Transduction
Transfection
|
IF |
41.846
|
Times Cited |
120
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
C.elegans |
tm1946
tm2943
tm1963 |