RRC ID 6395
著者 Phillips CM, Meng X, Zhang L, Chretien JH, Urnov FD, Dernburg AF.
タイトル Identification of chromosome sequence motifs that mediate meiotic pairing and synapsis in C. elegans.
ジャーナル Nat Cell Biol
Abstract Caenorhabditis elegans chromosomes contain specialized regions called pairing centres, which mediate homologous pairing and synapsis during meiosis. Four related proteins, ZIM-1, 2, 3 and HIM-8, associate with these sites and are required for their essential functions. Here we show that short sequence elements enriched in the corresponding chromosome regions selectively recruit these proteins in vivo. In vitro analysis using SELEX indicates that the binding specificity of each protein arises from a combination of two zinc fingers and an adjacent domain. Insertion of a cluster of recruiting motifs into a chromosome lacking its endogenous pairing centre is sufficient to restore homologous pairing, synapsis, crossover recombination and segregation. These findings help to illuminate how chromosome sites mediate essential aspects of meiotic chromosome dynamics.
巻・号 11(8)
ページ 934-42
公開日 2009-8-1
DOI 10.1038/ncb1904
PII ncb1904
PMID 19620970
PMC PMC4001799
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism Chromosome Pairing / genetics* Crossing Over, Genetic Disorders of Sex Development / genetics Female Genotype Immunohistochemistry In Situ Hybridization, Fluorescence Male Meiosis / genetics* Microscopy, Fluorescence RNA Interference SELEX Aptamer Technique Synaptonemal Complex / genetics Synaptonemal Complex / metabolism X Chromosome / genetics*
IF 20.042
引用数 76
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm574 tm511 tm611