論文 - 詳細
| RRC ID | 6402 |
|---|---|
| 著者 | Ruaud AF, Nilsson L, Richard F, Larsen MK, Bessereau JL, Tuck S. |
| タイトル | The C. elegans P4-ATPase TAT-1 regulates lysosome biogenesis and endocytosis. |
| ジャーナル | Traffic |
| Abstract |
P-type adenosine triphosphatases (ATPases) of the Drs2p family (P4-ATPases) are multipass transmembrane proteins required to generate and maintain phospholipid asymmetry in membrane bilayers. In Saccharomyces cerevisiae, several members of this family control distinct transport events within the endosomal and secretory pathways. Comparatively, little is known about the functions of P4-ATPases in multicellular organisms. In this study, we analyzed the role of the Caenorhabditis elegans Drs2p homologue transbilayer amphipath transporter (TAT)-1 in intracellular trafficking. tat-1 is expressed in many tissues including the intestine, the epidermis and the nervous system. In intestinal cells, tat-1 loss-of-function mutants accumulate large vacuoles of mixed endolysosomal identity positive for the lysosomal protein LMP-1. In addition, they lack the same class of storage granules as lmp-1 mutants, suggesting that part of the tat-1 phenotype might result from LMP-1 sequestration in an aberrant compartment. Epidermal cells mutant for tat-1 contain acidified giant hybrid multivesicular bodies probably corresponding to endolysosomal intermediate compartments or deficient lysosomes. Finally, TAT-1 is required for yolk uptake in oocytes and an early step of fluid-phase endocytosis in the intestine. Hence, TAT-1 is required at multiple steps of the endolysosomal pathway, at least in part by ensuring proper trafficking of cell-specific effector proteins. |
| 巻・号 | 10(1) |
| ページ | 88-100 |
| 公開日 | 2009-1-1 |
| DOI | 10.1111/j.1600-0854.2008.00844.x |
| PII | TRA844 |
| PMID | 18939953 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis* Female Gene Expression Regulation Humans Intestinal Mucosa / metabolism Lysosomes / enzymology* Lysosomes / ultrastructure Microscopy, Electron Mutation / genetics Organelle Biogenesis* Phospholipid Transfer Proteins / genetics Phospholipid Transfer Proteins / metabolism* Phylogeny Protein Transport Time Factors Vacuoles / metabolism |
| IF | 4.045 |
| 引用数 | 40 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1034 |