論文 - 詳細
| RRC ID | 6404 |
|---|---|
| 著者 | Meier B, Barber LJ, Liu Y, Shtessel L, Boulton SJ, Gartner A, Ahmed S. |
| タイトル | The MRT-1 nuclease is required for DNA crosslink repair and telomerase activity in vivo in Caenorhabditis elegans. |
| ジャーナル | EMBO J |
| Abstract |
The telomerase reverse transcriptase adds de novo DNA repeats to chromosome termini. Here we define Caenorhabditis elegans MRT-1 as a novel factor required for telomerase-mediated telomere replication and the DNA-damage response. MRT-1 is composed of an N-terminal domain homologous to the second OB-fold of POT1 telomere-binding proteins and a C-terminal SNM1 family nuclease domain, which confer single-strand DNA-binding and processive 3'-to-5' exonuclease activity, respectively. Furthermore, telomerase activity in vivo depends on a functional MRT-1 OB-fold. We show that MRT-1 acts in the same telomere replication pathway as telomerase and the 9-1-1 DNA-damage response complex. MRT-1 is dispensable for DNA double-strand break repair, but functions with the 9-1-1 complex to promote DNA interstrand cross-link (ICL) repair. Our data reveal MRT-1 as a dual-domain protein required for telomerase function and ICL repair, which raises the possibility that telomeres and ICL lesions may share a common feature that plays a critical role in de novo telomere repeat addition. |
| 巻・号 | 28(22) |
| ページ | 3549-63 |
| 公開日 | 2009-11-18 |
| DOI | 10.1038/emboj.2009.278 |
| PII | emboj2009278 |
| PMID | 19779462 |
| PMC | PMC2782091 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA Damage / physiology DNA Repair / genetics* DNA, Single-Stranded / metabolism Deoxyribonucleases / chemistry Deoxyribonucleases / genetics Deoxyribonucleases / metabolism Deoxyribonucleases / physiology* Enzyme Activation / genetics Molecular Sequence Data Protein Binding Protein Structure, Tertiary / genetics Sequence Homology, Amino Acid Telomerase / metabolism* Telomere / metabolism |
| IF | 9.889 |
| 引用数 | 26 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1354 tm899 |