RRC ID 6404
著者 Meier B, Barber LJ, Liu Y, Shtessel L, Boulton SJ, Gartner A, Ahmed S.
タイトル The MRT-1 nuclease is required for DNA crosslink repair and telomerase activity in vivo in Caenorhabditis elegans.
ジャーナル EMBO J
Abstract The telomerase reverse transcriptase adds de novo DNA repeats to chromosome termini. Here we define Caenorhabditis elegans MRT-1 as a novel factor required for telomerase-mediated telomere replication and the DNA-damage response. MRT-1 is composed of an N-terminal domain homologous to the second OB-fold of POT1 telomere-binding proteins and a C-terminal SNM1 family nuclease domain, which confer single-strand DNA-binding and processive 3'-to-5' exonuclease activity, respectively. Furthermore, telomerase activity in vivo depends on a functional MRT-1 OB-fold. We show that MRT-1 acts in the same telomere replication pathway as telomerase and the 9-1-1 DNA-damage response complex. MRT-1 is dispensable for DNA double-strand break repair, but functions with the 9-1-1 complex to promote DNA interstrand cross-link (ICL) repair. Our data reveal MRT-1 as a dual-domain protein required for telomerase function and ICL repair, which raises the possibility that telomeres and ICL lesions may share a common feature that plays a critical role in de novo telomere repeat addition.
巻・号 28(22)
ページ 3549-63
公開日 2009-11-18
DOI 10.1038/emboj.2009.278
PII emboj2009278
PMID 19779462
PMC PMC2782091
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA Damage / physiology DNA Repair / genetics* DNA, Single-Stranded / metabolism Deoxyribonucleases / chemistry Deoxyribonucleases / genetics Deoxyribonucleases / metabolism Deoxyribonucleases / physiology* Enzyme Activation / genetics Molecular Sequence Data Protein Binding Protein Structure, Tertiary / genetics Sequence Homology, Amino Acid Telomerase / metabolism* Telomere / metabolism
IF 9.889
引用数 26
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1354 tm899