| RRC ID |
64054
|
| 著者 |
Hisahara S, Iwahara N, Matsushita T, Suzuki S, Matsumura A, Fujikura M, Yokokawa K, Saito T, Manabe T, Kawamata J, Horio Y, Shimohama S.
|
| タイトル |
SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
SIRT1 is involved in the regulation of a variety of biological processes such as metabolism, stress response, autophagy and differentiation. Although progenitor cells of oligodendrocytes (OPCs) express high level of SIRT1, its function on differentiation is unknown. Because we have shown that SIRT1 plays a pivotal role in differentiation of neural precursor cells, we hypothesized that SIRT1 may also participate in the differentiation of oligodendrocytes (OLGs). We examined whether SIRT1 was expressed in two human oligodendrocyte cell lines: KG-1-C and MO 3.13 OLG. Transfection of cell lines with SIRT1-siRNA and SIRT2-siRNA promoted the extension of cellular processes. SIRT1-siRNA and SIRT2-siRNA increased acetyl-α-tubulin level, conversely, over expression of SIRTs resulted in decreased the ratio of acetyl-α-tubulin to α-tubulin. We also found knockdown of SIRT1 and SIRT2 induced overexpression of βIV-tubulin and tubulin polymerization promoting protein (TPPP) (OLG-specific cytoskeleton-related molecules) that distributed widely in cell bodies. Taken together, SIRT1 may play a role in oligodenroglial differentiation and myelinogenesis.
|
| 巻・号 |
546
|
| ページ |
7-14
|
| 公開日 |
2021-3-26
|
| DOI |
10.1016/j.bbrc.2021.01.095
|
| PII |
S0006-291X(21)00160-1
|
| PMID |
33556638
|
| MeSH |
Acetylation
Cell Differentiation / genetics
Cell Line
Cell Shape*
Cytoskeleton / metabolism*
Gene Expression Regulation*
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Oligodendroglia / cytology*
Oligodendroglia / metabolism*
RNA, Small Interfering / genetics
Sirtuin 1 / deficiency
Sirtuin 1 / genetics
Sirtuin 1 / metabolism*
Sirtuin 2 / genetics
Sirtuin 2 / metabolism
Tubulin / chemistry
Tubulin / genetics
Tubulin / metabolism
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
KG-1-C(RCB0270) |
