| RRC ID |
64064
|
| 著者 |
Matsuya Y, McMahon SJ, Butterworth KT, Naijo S, Nara I, Yachi Y, Saga R, Ishikawa M, Sato T, Date H, Prise KM.
|
| タイトル |
Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival.
|
| ジャーナル |
Phys Med Biol
|
| Abstract |
Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose to tumours under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field cells. However, the impact of hypoxia on radio-sensitivity under modulated radiation intensity remains to be fully clarified. Here, we investigate the impact of hypoxia on in-field and out-of-field radio-sensitivities using two types of cancer cells, DU145 and H1299. Using a nBIONIX hypoxic culture kit and a shielding technique to irradiate 50% of a cell culture flask, oxygen enhancement ratios (OERs) for double-strand breaks (DSB) and cell death endpoints were determined. These in vitro measurements indicate that hypoxia impacts out-of-field cells, although the hypoxic impacts on out-of-field cells for cell survival were dose-dependent and smaller compared to those for in-field and uniformly irradiated cells. These decreased radio-sensitivities of out-of-field cells were shown as a consistent tendency for both DSB and cell death endpoints, suggesting that radiation-induced intercellular communication is of importance in advanced radiotherapy dose-distributions such as with intensity-modulated radiotherapy.
|
| 巻・号 |
66(7)
|
| 公開日 |
2021-4-6
|
| DOI |
10.1088/1361-6560/abf011
|
| PMID |
33735839
|
| MeSH |
Cell Line, Tumor
Cell Survival
DNA Damage
Dose-Response Relationship, Radiation
Humans
Neoplasms* / radiotherapy
Oxygen
X-Rays
|
| IF |
2.883
|
| リソース情報 |
| ヒト・動物細胞 |
DU145(RCB2143) |
