論文 - 詳細
| RRC ID | 64064 |
|---|---|
| 著者 | Matsuya Y, McMahon SJ, Butterworth KT, Naijo S, Nara I, Yachi Y, Saga R, Ishikawa M, Sato T, Date H, Prise KM. |
| タイトル | Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival. |
| ジャーナル | Phys Med Biol |
| Abstract |
Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose to tumours under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field cells. However, the impact of hypoxia on radio-sensitivity under modulated radiation intensity remains to be fully clarified. Here, we investigate the impact of hypoxia on in-field and out-of-field radio-sensitivities using two types of cancer cells, DU145 and H1299. Using a nBIONIX hypoxic culture kit and a shielding technique to irradiate 50% of a cell culture flask, oxygen enhancement ratios (OERs) for double-strand breaks (DSB) and cell death endpoints were determined. These in vitro measurements indicate that hypoxia impacts out-of-field cells, although the hypoxic impacts on out-of-field cells for cell survival were dose-dependent and smaller compared to those for in-field and uniformly irradiated cells. These decreased radio-sensitivities of out-of-field cells were shown as a consistent tendency for both DSB and cell death endpoints, suggesting that radiation-induced intercellular communication is of importance in advanced radiotherapy dose-distributions such as with intensity-modulated radiotherapy. |
| 巻・号 | 66(7) |
| 公開日 | 2021-4-6 |
| DOI | 10.1088/1361-6560/abf011 |
| PMID | 33735839 |
| MeSH | Cell Line, Tumor Cell Survival DNA Damage Dose-Response Relationship, Radiation Humans Neoplasms* / radiotherapy Oxygen X-Rays |
| IF | 2.883 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | DU145(RCB2143) |