RRC ID 64078
著者 Aoyanagi T, Ikeya S, Kobayashi A, Kozaki A.
タイトル Gene Regulation via the Combination of Transcription Factors in the INDETERMINATE DOMAIN and GRAS Families.
ジャーナル Genes (Basel)
Abstract INDETERMINATE DOMAIN (IDD) family proteins are plant-specific transcription factors. Some Arabidopsis IDD (AtIDD) proteins regulate the expression of SCARECROW (SCR) by interacting with GRAS family transcription factors SHORT-ROOT (SHR) and SCR, which are involved in root tissue formation. Some AtIDD proteins regulate genes involved in the synthesis (GA3ox1) or signaling (SCL3) of gibberellic acid (GA) by interacting with DELLA proteins, a subfamily of the GRAS family. We analyzed the DNA binding properties and protein-protein interactions of select AtIDD proteins. We also investigated the transcriptional activity of the combination of AtIDD and GRAS proteins (AtIDD proteins combined with SHR and SCR or with REPRESSOR of ga1-3 (RGA)) on the promoters of SCR,SCL3, and GA3ox1 by conducting a transient assay using Arabidopsis culture cells. Our results showed that the SCR promoter could be activated by the IDD and RGA complexes and that the SCL3 and GA3ox1 promoters could be activated by the IDD, SHR, and SCR complexes, indicating the possibility that these complexes regulate and consequently coordinate the expression of genes involved in GA synthesis (GA3ox1), GA signaling (SCL3), and root formation (SCR).
巻・号 11(6)
公開日 2020-6-2
DOI 10.3390/genes11060613
PII genes11060613
PMID 32498388
PMC PMC7349898
MeSH Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis Proteins / genetics* Co-Repressor Proteins / genetics* DNA-Binding Proteins / genetics Gibberellins / metabolism Mixed Function Oxygenases / genetics* Multigene Family / genetics Promoter Regions, Genetic / genetics Repressor Proteins / genetics Transcription Factors / genetics
IF 3.759
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008