RRC ID 64081
著者 Weigt M, Gao Q, Ban H, He H, Mastrobuoni G, Kempa S, Chen W, Li F.
タイトル Rbm10 facilitates heterochromatin assembly via the Clr6 HDAC complex.
ジャーナル Epigenetics Chromatin
Abstract Splicing factors have recently been shown to be involved in heterochromatin formation, but their role in controlling heterochromatin structure and function remains poorly understood. In this study, we identified a fission yeast homologue of human splicing factor RBM10, which has been linked to TARP syndrome. Overexpression of Rbm10 in fission yeast leads to strong global intron retention. Rbm10 also interacts with splicing factors in a pattern resembling that of human RBM10, suggesting that the function of Rbm10 as a splicing regulator is conserved. Surprisingly, our deep-sequencing data showed that deletion of Rbm10 caused only minor effect on genome-wide gene expression and splicing. However, the mutant displays severe heterochromatin defects. Further analyses indicated that the heterochromatin defects in the mutant did not result from mis-splicing of heterochromatin factors. Our proteomic data revealed that Rbm10 associates with the histone deacetylase Clr6 complex and chromatin remodelers known to be important for heterochromatin silencing. Deletion of Rbm10 results in significant reduction of Clr6 in heterochromatin. Our work together with previous findings further suggests that different splicing subunits may play distinct roles in heterochromatin regulation.
巻・号 14(1)
ページ 8
公開日 2021-1-19
DOI 10.1186/s13072-021-00382-y
PII 10.1186/s13072-021-00382-y
PMID 33468217
PMC PMC7816512
MeSH Cell Cycle Proteins / metabolism Heterochromatin / genetics Histone Deacetylases / genetics Histone Deacetylases / metabolism Humans Proteomics RNA-Binding Proteins / genetics Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism
IF 4.237
リソース情報
酵母