RRC ID 6411
Author Minniti AN, Cataldo R, Trigo C, Vasquez L, Mujica P, Leighton F, Inestrosa NC, Aldunate R.
Title Methionine sulfoxide reductase A expression is regulated by the DAF-16/FOXO pathway in Caenorhabditis elegans.
Journal Aging Cell
Abstract The methionine sulfoxide reductase system has been implicated in aging and protection against oxidative stress. This conserved system reverses the oxidation of methionine residues within proteins. We analyzed one of the components of this system, the methionine sulfoxide reductase A gene, in Caenorhabditis elegans. We found that the msra-1 gene is expressed in most tissues, particularly in the intestine and the nervous system. Worms carrying a deletion of the msra-1 gene are more sensitive to oxidative stress, show chemotaxis and locomotory defects, and a 30% decrease in median survival. We established that msra-1 expression decreases during aging and is regulated by the DAF-16/FOXO3a transcription factor. The absence of this enzyme decreases median survival and affects oxidative stress resistance of long lived daf-2 worms. A similar effect of MSRA-1 absence in wild-type and daf-2 (where most antioxidant enzymes are activated) backgrounds, suggests that the lack of this member of the methionine repair system cannot be compensated by the general antioxidant response. Moreover, FOXO3a directly activates the human MsrA promoter in a cell culture system, implying that this could be a conserved mechanism of MsrA regulation. Our results suggest that repair of oxidative damage in proteins influences the rate at which tissues age. This repair mechanism, rather than the general decreased of radical oxygen species levels, could be one of the main determinants of organisms' lifespan.
Volume 8(6)
Pages 690-705
Published 2009-12-1
DOI 10.1111/j.1474-9726.2009.00521.x
PMID 19747232
MeSH 5' Untranslated Regions Aging Animals Behavior, Animal Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chemotaxis Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism* Gene Expression Regulation, Enzymologic* Humans Locomotion Methionine Sulfoxide Reductases Oxidative Stress Oxidoreductases / genetics Oxidoreductases / metabolism* Promoter Regions, Genetic Signal Transduction* Transcription Factors / genetics Transcription Factors / metabolism*
IF 7.346
Times Cited 49
C.elegans tm1421