| RRC ID |
6418
|
| Author |
Novelli JF, Chaudhary K, Canovas J, Benner JS, Madinger CL, Kelly P, Hodgkin J, Carlow CK.
|
| Title |
Characterization of the Caenorhabditis elegans UDP-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis.
|
| Journal |
Dev Biol
|
| Abstract |
Galactofuranose (Gal(f)), the furanoic form of d-galactose produced by UDP-galactopyranose mutases (UGMs), is present in surface glycans of some prokaryotes and lower eukaryotes. Absence of the Gal(f) biosynthetic pathway in vertebrates and its importance in several pathogens make UGMs attractive drug targets. Since the existence of Gal(f) in nematodes has not been established, we investigated the role of the Caenorhabditis elegans UGM homolog glf-1 in worm development. glf-1 mutants display significant late embryonic and larval lethality, and other phenotypes indicative of defective surface coat synthesis, the glycan-rich outermost layer of the nematode cuticle. The glf homolog from the protozoan Leishmania major partially complements C. elegans glf-1. glf-1 mutants rescued by L. major glf, which behave as glf-1 hypomorphs, display resistance to infection by Microbacterium nematophilum, a pathogen of rhabditid nematodes thought to bind to surface coat glycans. To confirm the presence of Gal(f) in C. elegans, we analyzed C. elegans nucleotide sugar pools using online electrospray ionization-mass spectrometry (ESI-MS). UDP-Gal(f) was detected in wild-type animals while absent in glf-1 deletion mutants. Our data indicate that Gal(f) likely has a pivotal role in maintenance of surface integrity in nematodes, supporting investigation of UGM as a drug target in parasitic species.
|
| Volume |
335(2)
|
| Pages |
340-55
|
| Published |
2009-11-15
|
| DOI |
10.1016/j.ydbio.2009.09.010
|
| PII |
S0012-1606(09)01179-8
|
| PMID |
19751718
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Genetically Modified
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / microbiology
Caenorhabditis elegans Proteins / biosynthesis*
Galactose / metabolism*
Gene Knockout Techniques
Gram-Positive Bacteria / pathogenicity
Intramolecular Transferases / chemistry
Intramolecular Transferases / genetics
Intramolecular Transferases / metabolism*
Molecular Sequence Data
Sequence Homology, Amino Acid
Spectrometry, Mass, Electrospray Ionization
|
| IF |
2.896
|
| Times Cited |
29
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| C.elegans |
tm2621
tm2412 |
