RRC ID 6425
Author Parry JM, Velarde NV, Lefkovith AJ, Zegarek MH, Hang JS, Ohm J, Klancer R, Maruyama R, Druzhinina MK, Grant BD, Piano F, Singson A.
Title EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans.
Journal Curr. Biol.
Abstract The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.
Volume 19(20)
Pages 1752-7
Published 2009-11-3
DOI 10.1016/j.cub.2009.09.015
PII S0960-9822(09)01692-3
PMID 19879147
PMC PMC2783797
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Chitin Synthase / analysis Chitin Synthase / genetics Chitin Synthase / physiology Cytoplasm / metabolism Embryonic Development / genetics* Meiosis / physiology* Molecular Sequence Data Oocytes / cytology Oocytes / growth & development Oocytes / metabolism Protein Transport Protein-Tyrosine Kinases / analysis Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / physiology Sequence Alignment
IF 9.193
Times Cited 39
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1508