論文 - 詳細
| RRC ID | 64256 |
|---|---|
| 著者 | Tanaka Y, Nanasato Y, Omura K, Endoh K, Kawano T, Iwasaki T. |
| タイトル | Direct protein delivery into intact plant cells using polyhistidine peptides. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Polyhistidine peptides (PHPs), sequences comprising only histidine residues (>His8), are effective cell-penetrating peptides for plant cells. Using PHP-fusion proteins, we aimed to deliver proteins into cultured plant cells from Nicotiana tabacum, Oryza sativa, and Cryptomeria japonica. Co-cultivation of cultured cells with fusion proteins combining maltose-binding protein (MBP), red fluorescent protein (RFP), and various PHPs (MBP-RFP-His8-His20) in one polypeptide showed the cellular uptake of fusion proteins in all plant cell lines. Maximum intracellular fluorescence was shown in MBP-RFP-His20. Further, adenylate cyclase (CyaA), a synthase of cyclic adenosine monophosphate (cAMP) activated by cytosolic calmodulin, was used as a reporter for protein delivery in living cells. A fusion protein combining MBP, RFP, CyaA, and His20 (MBP-RFP-CyaA-His20) was delivered into plant cells and increased intracellular fluorescence and cAMP production in all cell lines. The present study demonstrates that PHPs are effective carriers of proteins into the intracellular space of various cultured plant cells. |
| 巻・号 | 85(6) |
| ページ | 1405-1414 |
| 公開日 | 2021-5-25 |
| DOI | 10.1093/bbb/zbab055 |
| PII | 6206858 |
| PMID | 33791772 |
| MeSH | Biological Transport Cell Line Cell Membrane / metabolism Drug Carriers / chemistry* Drug Carriers / metabolism* Histidine / chemistry* Peptides / chemistry* Peptides / metabolism* Plant Cells / metabolism* Protein Transport Recombinant Fusion Proteins / chemistry* Recombinant Fusion Proteins / metabolism |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 rpc00031 |