論文 - 詳細
| RRC ID | 64287 |
|---|---|
| 著者 | Izumi Y, Furuse K, Furuse M. |
| タイトル | The novel membrane protein Hoka regulates septate junction organization and stem cell homeostasis in the Drosophila gut. |
| ジャーナル | J Cell Sci |
| Abstract |
Smooth septate junctions (sSJs) regulate the paracellular transport in the intestinal tract in arthropods. In Drosophila, the organization and physiological function of sSJs are regulated by at least three sSJ-specific membrane proteins: Ssk, Mesh and Tsp2A. Here, we report a novel sSJ membrane protein, Hoka, which has a single membrane-spanning segment with a short extracellular region, and a cytoplasmic region with Tyr-Thr-Pro-Ala motifs. The larval midgut in hoka mutants shows a defect in sSJ structure. Hoka forms a complex with Ssk, Mesh and Tsp2A, and is required for the correct localization of these proteins to sSJs. Knockdown of hoka in the adult midgut leads to intestinal barrier dysfunction and stem cell overproliferation. In hoka-knockdown midguts, aPKC is upregulated in the cytoplasm and the apical membrane of epithelial cells. The depletion of aPKC and yki in hoka-knockdown midguts results in reduced stem cell overproliferation. These findings indicate that Hoka cooperates with the sSJ proteins Ssk, Mesh and Tsp2A to organize sSJs, and is required for maintaining intestinal stem cell homeostasis through the regulation of aPKC and Yki activities in the Drosophila midgut. |
| 巻・号 | 134(6) |
| 公開日 | 2021-3-26 |
| DOI | 10.1242/jcs.257022 |
| PII | jcs.257022 |
| PMID | 33589496 |
| MeSH | Animals Drosophila* Drosophila Proteins* / genetics Drosophila melanogaster / genetics Homeostasis Membrane Proteins / genetics Stem Cells Tetraspanins |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 13704R-1 TBX-0002 TBX-0004 TBX-0007 CAS-0002 TBX-0010 DGRC#112001 DGRC#108851 |