RRC ID 64327
著者 Omori H, Otsu M, Nogami H, Shibata M.
タイトル Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.
ジャーナル PLoS One
Abstract Cells have a regulatory mechanism known as heat shock (HS) response, which induces the expression of HS genes and proteins in response to heat and other cellular stresses. Exposure to moderate HS results in beneficial effects, such as thermotolerance and promotes survival, whereas excessive HS causes cell death. The effect of HS on cells depends on both exogenous factors, including the temperature and duration of heat application, and endogenous factors, such as the degree of cell differentiation. Neural stem cells (NSCs) can self-renew and differentiate into neurons and glial cells, but the changes in the HS response of symmetrically proliferating NSCs in culture are unclear. We evaluated the HS response of homogeneous proliferating NSCs derived from mouse embryonic stem cells during the proliferative phase and its effect on survival and cell death in vitro. The number of adherent cells and the expression ratios of HS protein (Hsp)40 and Hsp70 genes after exposure to HS for 20 min at temperatures above 43°C significantly increased with the extension of the culture period before exposure to HS. In contrast, caspase activity was significantly decreased by extension of the culture period before exposure to HS and suppressed the decrease in cell viability. These results suggest that the culture period before HS remarkably affects the HS response, influencing the expression of HS genes and cell survival of proliferating NSCs in culture.
巻・号 16(4)
ページ e0249954
公開日 2021-1-1
DOI 10.1371/journal.pone.0249954
PII PONE-D-20-29678
PMID 33852623
PMC PMC8046196
MeSH Animals Caspase 3 / genetics Caspase 3 / metabolism Cell Differentiation Cell Proliferation Cell Self Renewal Cell Survival Cells, Cultured HSP27 Heat-Shock Proteins / genetics HSP27 Heat-Shock Proteins / metabolism HSP40 Heat-Shock Proteins / genetics HSP40 Heat-Shock Proteins / metabolism* HSP70 Heat-Shock Proteins / genetics HSP70 Heat-Shock Proteins / metabolism* Mice Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / metabolism Neural Stem Cells / cytology Neural Stem Cells / metabolism* Neuroglia / cytology Neuroglia / metabolism Neurons / cytology Neurons / metabolism Temperature
IF 2.74
リソース情報
ヒト・動物細胞 B6J-S1^(UTR)(AES0140)