Reference - Detail
RRC ID | 6433 |
---|---|
Author | Voutev R, Keating R, Hubbard EJ, Vallier LG. |
Title | Characterization of the Caenorhabditis elegans Islet LIM-homeodomain ortholog, lim-7. |
Journal | FEBS Lett |
Abstract |
lim-7 is one of seven Caenorhabditis elegans LIM-homeodomain (LIM-HD)-encoding genes and the sole Islet ortholog. LIM-HD transcription factors, including Islets, function in neuronal and non-neuronal development across diverse phyla. Our results show that a lim-7 deletion allele causes early larval lethality with terminal phenotypes including uncoordination, detached pharynx, constipation and morphological defects. A lim-7(+) transgene rescues lethality but not adult sterility. A lim-7(+) reporter in the full genomic context is expressed in all gonadal sheath cells, URA neurons, and additional cells in the pharyngeal region. Finally, we identify a 45-bp regulatory element in the first intron that is necessary and sufficient for lim-7 gonadal sheath expression. |
Volume | 583(2) |
Pages | 456-64 |
Published | 2009-1-22 |
DOI | 10.1016/j.febslet.2008.12.046 |
PII | S0014-5793(08)01038-7 |
PMID | 19116151 |
PMC | PMC2719984 |
MeSH | Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans / anatomy & histology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental Genes, Essential Genes, Lethal Gonads / anatomy & histology Gonads / growth & development Gonads / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* LIM-Homeodomain Proteins Molecular Sequence Data Phenotype RNA Interference Regulatory Elements, Transcriptional |
IF | 3.057 |
Times Cited | 18 |
WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
C.elegans | tm674 |