RRC ID 64417
Author Zhu C, Zheng XF, Yang YH, Li B, Wang YR, Jiang SD, Jiang LS.
Title LGR4 acts as a key receptor for R-spondin 2 to promote osteogenesis through Wnt signaling pathway.
Journal Cell Signal
Abstract R-spondin proteins are identified as secreted agonists of the canonical Wnt/β-catenin signaling pathway, and leucine-rich repeat-containing G-protein-coupled receptors (LGR) are recognized as R-spondin receptors. The potential role of R-spondin 2 (Rspo2) and LGR4 in mediating osteogenesis remains poorly understood. In our in vitro experiments, we found that Rspo2 could promote osteogenesis through activating the Wnt signaling pathway in MC3T3-E1 cells. However, this effect of Rsop2 disappeared in the cells with functional disruption of LGR4. Meanwhile, Rspo2 significantly inhibited osteoclastogenesis and this effect of Rspo2 was dependent on the presence of osteoblasts with normal function of LGR4. In our in vivo experiments, we found that application of exogenous Rspo2 rescued the bone loss and improved the microarchitecture of bone in OVX mice. Rspo2 could be a positive regulator of bone metabolism through activating the canonical Wnt/β-catenin signaling, and LGR4 acted as a key receptor for Rspo2 to promote osteogenesis.
Volume 28(8)
Pages 989-1000
Published 2016-8-1
DOI 10.1016/j.cellsig.2016.04.010
PII S0898-6568(16)30094-8
PMID 27140682
MeSH Animals Bone Resorption / metabolism Bone Resorption / pathology Calcification, Physiologic Cell Differentiation Cell Line Female Gene Silencing Low Density Lipoprotein Receptor-Related Protein-5 / metabolism Mice, Inbred C57BL Osteoblasts / cytology Osteoblasts / metabolism Osteoclasts / cytology Osteoclasts / metabolism Osteogenesis* Ovariectomy Protein Stability Receptors, G-Protein-Coupled / metabolism* Thrombospondins / metabolism* Wnt Signaling Pathway* beta Catenin / metabolism
IF 3.968
Human and Animal Cells MC3T3-E1(RCB1126)