RRC ID 64446
Author Ishizuka K, Fujii W, Azuma N, Mizobuchi H, Morimoto A, Sanjoba C, Matsumoto Y, Goto Y.
Title Pathological roles of MRP14 in anemia and splenomegaly during experimental visceral leishmaniasis.
Journal PLoS Negl Trop Dis
Abstract Myeloid-related protein 14 (MRP14) belongs to the S100 calcium-binding protein family and is expressed in neutrophils and inflammatory macrophages. Increase in the number of MRP14+ cells or serum level of MRP14 is associated with various diseases such as autoimmune diseases and infectious diseases, suggesting the involvement of the molecule in pathogenesis of those diseases. In this study, to examine the pathological involvement of MRP14 during cutaneous and visceral leishmaniasis, wild-type (WT) and MRP14 knockout (MRP14KO) mice were infected with Leishmania major and L. donovani. Increase in the number of MRP14+ cells at the infection sites in wild-type mice was commonly found in the skin during L. major infection as well as the spleen and liver during L. donovani infection. In contrast, the influence of MRP14 to the pathology seemed different between the two infections. MRP14 depletion exacerbated the lesion development and ulcer formation in L. major infection. On the other hand, the depletion improved anemia and splenomegaly but not hepatomegaly at 24 weeks of L. donovani infection. These results suggest that, distinct from its protective role in CL, MRP14 is involved in exacerbation of some symptoms during VL.
Volume 14(1)
Pages e0008020
Published 2020-1-1
DOI 10.1371/journal.pntd.0008020
PII PNTD-D-19-01202
PMID 31961866
PMC PMC6994150
MeSH Anemia / genetics Anemia / metabolism* Anemia / parasitology Anemia / pathology* Animals Calgranulin B / genetics Calgranulin B / metabolism* Female Humans Leishmania donovani / physiology Leishmania major / physiology Leishmaniasis, Visceral / genetics Leishmaniasis, Visceral / metabolism* Leishmaniasis, Visceral / parasitology Leishmaniasis, Visceral / pathology* Liver / metabolism Liver / parasitology Liver / pathology Male Mice Mice, Inbred BALB C Mice, Knockout Spleen / metabolism Spleen / parasitology Spleen / pathology Splenomegaly / genetics Splenomegaly / metabolism* Splenomegaly / parasitology Splenomegaly / pathology*
IF 3.885
Pathogenic eukaryotic microorganisms Leishmania donovani D10 (Ld003)