RRC ID 64452
Author Sun H, Nishioka T, Hiramatsu S, Kondo S, Amano M, Kaibuchi K, Ichinose T, Tanimoto H.
Title Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila.
Journal J Neurosci
Abstract In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.SIGNIFICANCE STATEMENT Dopaminergic input to the Kenyon cells (KCs) is pivotal to stabilize memory in Drosophila This process is mediated by dopamine receptors like Dop1R2. Nevertheless, little is known for its underlying molecular mechanism. Here we show that the Raf/MAPK pathway is specifically engaged in appetitive long-term memory in KCs. With combined biochemical and behavioral experiments, we reveal that activation of the Raf/MAPK pathway is regulated through Dop1R2, shedding light on how dopamine modulates intracellular signaling for memory stabilization.
Volume 40(14)
Pages 2935-2942
Published 2020-4-1
DOI 10.1523/JNEUROSCI.1572-19.2020
PII JNEUROSCI.1572-19.2020
PMID 32102921
PMC PMC7117890
MeSH Animals Appetitive Behavior / physiology* Drosophila Drosophila Proteins / metabolism* Extracellular Signal-Regulated MAP Kinases / metabolism Female Male Memory, Long-Term / physiology* Mushroom Bodies / physiology Neurons / metabolism* Receptors, Dopamine D1 / metabolism* Signal Transduction / physiology* raf Kinases / metabolism
IF 5.674
Drosophila DGRC#106635