論文 - 詳細
| RRC ID | 64452 |
|---|---|
| 著者 | Sun H, Nishioka T, Hiramatsu S, Kondo S, Amano M, Kaibuchi K, Ichinose T, Tanimoto H. |
| タイトル | Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila. |
| ジャーナル | J Neurosci |
| Abstract |
In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.SIGNIFICANCE STATEMENT Dopaminergic input to the Kenyon cells (KCs) is pivotal to stabilize memory in Drosophila This process is mediated by dopamine receptors like Dop1R2. Nevertheless, little is known for its underlying molecular mechanism. Here we show that the Raf/MAPK pathway is specifically engaged in appetitive long-term memory in KCs. With combined biochemical and behavioral experiments, we reveal that activation of the Raf/MAPK pathway is regulated through Dop1R2, shedding light on how dopamine modulates intracellular signaling for memory stabilization. |
| 巻・号 | 40(14) |
| ページ | 2935-2942 |
| 公開日 | 2020-4-1 |
| DOI | 10.1523/JNEUROSCI.1572-19.2020 |
| PII | JNEUROSCI.1572-19.2020 |
| PMID | 32102921 |
| PMC | PMC7117890 |
| MeSH | Animals Appetitive Behavior / physiology* Drosophila Drosophila Proteins / metabolism* Extracellular Signal-Regulated MAP Kinases / metabolism Female Male Memory, Long-Term / physiology* Mushroom Bodies / physiology Neurons / metabolism* Receptors, Dopamine D1 / metabolism* Signal Transduction / physiology* raf Kinases / metabolism |
| IF | 5.674 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#106635 |