論文 - 詳細
| RRC ID | 64455 |
|---|---|
| 著者 | Iki T, Takami M, Kai T. |
| タイトル | Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs during Sperm Maturation in Drosophila. |
| ジャーナル | Cell Rep |
| Abstract |
In gene silencing, Hsp90 chaperone machinery assists Argonaute (Ago) binding and unwinding of silencing small RNA (sRNA) duplexes. This enables the formation of effector RNA-induced silencing complex (RISC) that often displays cargo preferences. Hence, in Drosophila, microRNAs (miRNAs) and small-interfering RNAs (siRNAs) are differentially sorted into Ago1-RISC and Ago2-RISC, respectively. Here, we identify fly Cyclophilin 40 (Cyp40) as a testis-specialized Hsp90 co-chaperone essential for spermatogenesis and for modulating Ago2-RISC formation. We show that testis-distinctive Ago-sorting and strand-selection mechanisms accumulate a unique set of miRNAs on Ago2. Cyp40 interacts with duplex-incorporating Ago2 through Hsp90 in vitro and selectively promotes the build-up of Ago2-bound miRNAs, but not endogenous siRNAs, in vivo. Moreover, one of Cyp40-dependent Ago2-sorted miRNAs is required for late spermatogenesis, unraveling the physiological relevance of the unconventional yet conserved Drosophila miRNA-Ago2 sorting pathway. Collectively, these results identify RISC-regulatory roles for Hsp90 machinery and, more generally, highlight the tissue-specific adaptation of sRNA pathways through chaperone diversification. |
| 巻・号 | 33(6) |
| ページ | 108380 |
| 公開日 | 2020-11-10 |
| DOI | 10.1016/j.celrep.2020.108380 |
| PII | S2211-1247(20)31369-3 |
| PMID | 33176138 |
| MeSH | Animals Argonaute Proteins / metabolism* Cyclophilin D / metabolism* Drosophila Drosophila Proteins / metabolism* Gene Silencing MicroRNAs / metabolism* Sperm Maturation / physiology* |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109173 DGRC#116356 DGRC#150442 DGRC#116390 DGRC#150028 DGRC#116331 DGRC#116368 |