RRC ID 64455
著者 Iki T, Takami M, Kai T.
タイトル Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs during Sperm Maturation in Drosophila.
ジャーナル Cell Rep
Abstract In gene silencing, Hsp90 chaperone machinery assists Argonaute (Ago) binding and unwinding of silencing small RNA (sRNA) duplexes. This enables the formation of effector RNA-induced silencing complex (RISC) that often displays cargo preferences. Hence, in Drosophila, microRNAs (miRNAs) and small-interfering RNAs (siRNAs) are differentially sorted into Ago1-RISC and Ago2-RISC, respectively. Here, we identify fly Cyclophilin 40 (Cyp40) as a testis-specialized Hsp90 co-chaperone essential for spermatogenesis and for modulating Ago2-RISC formation. We show that testis-distinctive Ago-sorting and strand-selection mechanisms accumulate a unique set of miRNAs on Ago2. Cyp40 interacts with duplex-incorporating Ago2 through Hsp90 in vitro and selectively promotes the build-up of Ago2-bound miRNAs, but not endogenous siRNAs, in vivo. Moreover, one of Cyp40-dependent Ago2-sorted miRNAs is required for late spermatogenesis, unraveling the physiological relevance of the unconventional yet conserved Drosophila miRNA-Ago2 sorting pathway. Collectively, these results identify RISC-regulatory roles for Hsp90 machinery and, more generally, highlight the tissue-specific adaptation of sRNA pathways through chaperone diversification.
巻・号 33(6)
ページ 108380
公開日 2020-11-10
DOI 10.1016/j.celrep.2020.108380
PII S2211-1247(20)31369-3
PMID 33176138
MeSH Animals Argonaute Proteins / metabolism* Cyclophilin D / metabolism* Drosophila Drosophila Proteins / metabolism* Gene Silencing MicroRNAs / metabolism* Sperm Maturation / physiology*
IF 8.109
リソース情報
ショウジョウバエ DGRC#109173 DGRC#116356 DGRC#150442 DGRC#116390 DGRC#150028 DGRC#116331 DGRC#116368