RRC ID 64461
著者 Rust K, Byrnes LE, Yu KS, Park JS, Sneddon JB, Tward AD, Nystul TG.
タイトル A single-cell atlas and lineage analysis of the adult Drosophila ovary.
ジャーナル Nat Commun
Abstract The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.
巻・号 11(1)
ページ 5628
公開日 2020-11-6
DOI 10.1038/s41467-020-19361-0
PII 10.1038/s41467-020-19361-0
PMID 33159074
PMC PMC7648648
MeSH Animals Cell Lineage Drosophila / cytology* Drosophila / genetics Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Female Ovarian Follicle / cytology Ovarian Follicle / metabolism Ovary / cytology* Ovary / metabolism Single-Cell Analysis
IF 12.121
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ショウジョウバエ DGRC#112322