| RRC ID |
64461
|
| 著者 |
Rust K, Byrnes LE, Yu KS, Park JS, Sneddon JB, Tward AD, Nystul TG.
|
| タイトル |
A single-cell atlas and lineage analysis of the adult Drosophila ovary.
|
| ジャーナル |
Nat Commun
|
| Abstract |
The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.
|
| 巻・号 |
11(1)
|
| ページ |
5628
|
| 公開日 |
2020-11-6
|
| DOI |
10.1038/s41467-020-19361-0
|
| PII |
10.1038/s41467-020-19361-0
|
| PMID |
33159074
|
| PMC |
PMC7648648
|
| MeSH |
Animals
Cell Lineage
Drosophila / cytology*
Drosophila / genetics
Drosophila / metabolism
Drosophila Proteins / genetics
Drosophila Proteins / metabolism
Female
Ovarian Follicle / cytology
Ovarian Follicle / metabolism
Ovary / cytology*
Ovary / metabolism
Single-Cell Analysis
|
| IF |
12.121
|
| リソース情報 |
| ショウジョウバエ |
DGRC#112322 |
