RRC ID 64468
Author Bajusz C, Kristó I, Abonyi C, Venit T, Vedelek V, Lukácsovich T, Farkas A, Borkúti P, Kovács Z, Bajusz I, Marton A, Vizler C, Lipinszki Z, Sinka R, Percipalle P, Vilmos P.
Title The nuclear activity of the actin-binding Moesin protein is necessary for gene expression in Drosophila.
Journal FEBS J
Abstract Ezrin-Radixin-Moesin (ERM) proteins play an essential role in the cytoplasm by cross-linking actin filaments with plasma membrane proteins. Research has identified the nuclear localization of ERMs, as well as the involvement of a single Drosophila ERM protein, Moesin, in nuclear mRNA exports. However, the question of how important the nuclear activity of ERM proteins are for the life of an organism has so far not been explored. Here, we present the first attempt to reveal the in vivo relevance of nuclear localization of Moesin in Drosophila. With the help of a nuclear export signal, we decreased the amount of Moesin in the nuclei of the animals. Furthermore, we observed various developmental defects, demonstrating the importance of ERM function in the nucleus for the first time. Transcriptome analysis of the mutant flies revealed that the lack of nuclear Moesin function leads to expression changes in nearly 700 genes, among them heat-shock genes. This result together with additional findings revealed that in Drosophila the expression of protein chaperones requires the nuclear functions of Moesin. DATABASE: GEO accession number: GSE155778.
Volume 288(16)
Pages 4812-4832
Published 2021-8-1
DOI 10.1111/febs.15779
PMID 33606336
MeSH Actins / genetics Actins / metabolism Animals Cell Nucleus / metabolism Drosophila Gene Expression Regulation / genetics Membrane Proteins / genetics Membrane Proteins / metabolism*
IF 4.392
Drosophila DGRC#108358