論文 - 詳細
| RRC ID | 6449 |
|---|---|
| 著者 | Geng X, Zhou QH, Kage-Nakadai E, Shi Y, Yan N, Mitani S, Xue D. |
| タイトル | Caenorhabditis elegans caspase homolog CSP-2 inhibits CED-3 autoactivation and apoptosis in germ cells. |
| ジャーナル | Cell Death Differ |
| Abstract |
In Caenorhabditis elegans, apoptosis in germ cells is mediated by the same core apoptotic machinery that controls apoptosis in somatic cells. These include the CED-3 caspase, the CED-3 activator CED-4, and the cell death inhibitor CED-9. However, germline apoptosis also differs from somatic apoptosis in its regulation. We found that CSP-3, a caspase homolog that blocks CED-3 autoactivation and apoptosis in somatic cells, does not affect apoptosis in germ cells. Interestingly, the second C. elegans caspase homolog, CSP-2, shares sequence similarity to both catalytic subunits of the CED-3 caspase, and surprisingly, contains a stretch of sequence that is almost identical to that of CSP-3. Unlike CSP-3 that acts specifically in somatic cells, loss of CSP-2 causes increased apoptosis only in germ cells, suggesting that CSP-2 is a germ cell-specific apoptosis inhibitor. Moreover, like CSP-3, CSP-2 associates with the CED-3 zymogen and inhibits its autoactivation, but does not inhibit CED-4-induced CED-3 activation or the activity of the activated CED-3 protease. Thus, two different C. elegans caspase homologs use the same mechanism to prevent caspase autoactivation and apoptosis in different tissues, suggesting that this could be a generally applicable strategy for regulating caspase activation and apoptosis. |
| 巻・号 | 16(10) |
| ページ | 1385-94 |
| 公開日 | 2009-10-1 |
| DOI | 10.1038/cdd.2009.88 |
| PII | cdd200988 |
| PMID | 19575016 |
| PMC | PMC2743765 |
| MeSH | Amino Acid Sequence Animals Apoptosis* Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Caspase 2 / chemistry Caspase 2 / genetics Caspase 2 / metabolism* Caspases / chemistry Caspases / metabolism* Germ Cells / cytology* Molecular Sequence Data Protein Structure, Tertiary Sequence Alignment Sequence Homology, Amino Acid |
| IF | 10.717 |
| 引用数 | 20 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2858 tm3077 tm2260 |