論文 - 詳細
| RRC ID | 64490 |
|---|---|
| 著者 | Li TN, Chen YJ, Lu TY, Wang YT, Lin HC, Yao CK. |
| タイトル | A positive feedback loop between Flower and PI(4,5)P2 at periactive zones controls bulk endocytosis in Drosophila. |
| ジャーナル | Elife |
| Abstract |
Synaptic vesicle (SV) endocytosis is coupled to exocytosis to maintain SV pool size and thus neurotransmitter release. Intense stimulation induces activity-dependent bulk endocytosis (ADBE) to recapture large quantities of SV constituents in large endosomes from which SVs reform. How these consecutive processes are spatiotemporally coordinated remains unknown. Here, we show that Flower Ca2+ channel-dependent phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) compartmentalization governs control of these processes in Drosophila. Strong stimuli trigger PI(4,5)P2 microdomain formation at periactive zones. Upon exocytosis, Flower translocates from SVs to periactive zones, where it increases PI(4,5)P2 levels via Ca2+ influxes. Remarkably, PI(4,5)P2 directly enhances Flower channel activity, thereby establishing a positive feedback loop for PI(4,5)P2 microdomain compartmentalization. PI(4,5)P2 microdomains drive ADBE and SV reformation from bulk endosomes. PI(4,5)P2 further retrieves Flower to bulk endosomes, terminating endocytosis. We propose that the interplay between Flower and PI(4,5)P2 is the crucial spatiotemporal cue that couples exocytosis to ADBE and subsequent SV reformation. |
| 巻・号 | 9 |
| 公開日 | 2020-12-10 |
| DOI | 10.7554/eLife.60125 |
| PII | 60125 |
| PMID | 33300871 |
| PMC | PMC7748424 |
| MeSH | Animals Calcium Channels / metabolism* Drosophila Drosophila Proteins / metabolism* Endocytosis / physiology* Feedback, Physiological / physiology Neuromuscular Junction / metabolism Phosphatidylinositol Phosphates / metabolism* Presynaptic Terminals / metabolism Synaptic Transmission / physiology Synaptic Vesicles / metabolism* |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#108068 |