RRC ID 6450
Author Gu W, Shirayama M, Conte D Jr, Vasale J, Batista PJ, Claycomb JM, Moresco JJ, Youngman EM, Keys J, Stoltz MJ, Chen CC, Chaves DA, Duan S, Kasschau KD, Fahlgren N, Yates JR 3rd, Mitani S, Carrington JC, Mello CC.
Title Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline.
Journal Mol. Cell
Abstract Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the Dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that, in the germline, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germline nuage structures called P granules. WAGO-1 silences certain genes, transposons, pseudogenes, and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest additional regulatory functions for small RNAs.
Volume 36(2)
Pages 231-44
Published 2009-10-23
DOI 10.1016/j.molcel.2009.09.020
PII S1097-2765(09)00678-9
PMID 19800275
PMC PMC2776052
MeSH Alleles Amino Acid Sequence Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Genome / genetics* Germ Cells / metabolism* Models, Genetic Molecular Sequence Data Phylogeny Protein Binding Protein Structure, Tertiary RNA Interference RNA Replicase / metabolism RNA, Helminth / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism* Sequence Analysis, RNA Temperature
IF 14.248
Times Cited 171
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1217 tm1120 tm1637 tm1019 tm2686 tm1113 tm1414