| RRC ID |
6450
|
| Author |
Gu W, Shirayama M, Conte D Jr, Vasale J, Batista PJ, Claycomb JM, Moresco JJ, Youngman EM, Keys J, Stoltz MJ, Chen CC, Chaves DA, Duan S, Kasschau KD, Fahlgren N, Yates JR 3rd, Mitani S, Carrington JC, Mello CC.
|
| Title |
Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline.
|
| Journal |
Mol Cell
|
| Abstract |
Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the Dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that, in the germline, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germline nuage structures called P granules. WAGO-1 silences certain genes, transposons, pseudogenes, and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest additional regulatory functions for small RNAs.
|
| Volume |
36(2)
|
| Pages |
231-44
|
| Published |
2009-10-23
|
| DOI |
10.1016/j.molcel.2009.09.020
|
| PII |
S1097-2765(09)00678-9
|
| PMID |
19800275
|
| PMC |
PMC2776052
|
| MeSH |
Alleles
Amino Acid Sequence
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Genome / genetics*
Germ Cells / metabolism*
Models, Genetic
Molecular Sequence Data
Phylogeny
Protein Binding
Protein Structure, Tertiary
RNA Interference
RNA Replicase / metabolism
RNA, Helminth / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism*
Sequence Analysis, RNA
Temperature
|
| IF |
14.548
|
| Times Cited |
232
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1217
tm1120
tm1637
tm1019
tm2686
tm1113
tm1414 |
