RRC ID 64558
Author Nakayama M, Nakamura A, Hondou T, Miyata H.
Title Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field.
Journal Int J Radiat Biol
Abstract PURPOSE:Synergistic effects between cellular oxidative stress and magnetic fields may explain the adverse biological effects of 50/60 Hz magnetic fields. To determine whether this hypothesis holds in macrophage RAW264 cells, we measured DNA single-strand breaks (SSB), cell viability, and nitric oxide (NO) production in cells with or without exposure to 0.5-mT, 50-Hz magnetic fields for 24 h and with or without simultaneous stimulation via the bacterial endotoxin, lipopolysaccharide (LPS).
MATERIALS AND METHODS:Macrophages stimulated with 10 ng/ml LPS for 1 h were exposed to or not exposed to a magnetic field and were then subjected to (1) the alkaline comet assay to measure SSBs, (2) trypan-blue exclusion assay for cell viability, and (3) measurements of NO for evaluation of oxidative stress.
RESULTS:The 50-Hz magnetic field enhanced DNA SSB and decreased cell viability only in the LPS-stimulated macrophages in which NO production was greatly enhanced. The magnetic field alone did not alter NO production.
CONCLUSION:Co-stimulation of the cell with LPS and a 50-Hz magnetic field promoted SSB and lowered cell viability, but these were not mediated by LPS-induced NO production.
Volume 92(10)
Pages 583-9
Published 2016-10-1
DOI 10.1080/09553002.2016.1206224
PMID 27430265
Resource
Human and Animal Cells RAW 264(RCB0535)