論文 - 詳細
| RRC ID | 64558 |
|---|---|
| 著者 | Nakayama M, Nakamura A, Hondou T, Miyata H. |
| タイトル | Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:Synergistic effects between cellular oxidative stress and magnetic fields may explain the adverse biological effects of 50/60 Hz magnetic fields. To determine whether this hypothesis holds in macrophage RAW264 cells, we measured DNA single-strand breaks (SSB), cell viability, and nitric oxide (NO) production in cells with or without exposure to 0.5-mT, 50-Hz magnetic fields for 24 h and with or without simultaneous stimulation via the bacterial endotoxin, lipopolysaccharide (LPS). MATERIALS AND METHODS:Macrophages stimulated with 10 ng/ml LPS for 1 h were exposed to or not exposed to a magnetic field and were then subjected to (1) the alkaline comet assay to measure SSBs, (2) trypan-blue exclusion assay for cell viability, and (3) measurements of NO for evaluation of oxidative stress. RESULTS:The 50-Hz magnetic field enhanced DNA SSB and decreased cell viability only in the LPS-stimulated macrophages in which NO production was greatly enhanced. The magnetic field alone did not alter NO production. CONCLUSION:Co-stimulation of the cell with LPS and a 50-Hz magnetic field promoted SSB and lowered cell viability, but these were not mediated by LPS-induced NO production. |
| 巻・号 | 92(10) |
| ページ | 583-9 |
| 公開日 | 2016-10-1 |
| DOI | 10.1080/09553002.2016.1206224 |
| PMID | 27430265 |
| MeSH | Animals Cell Survival / drug effects* Cell Survival / radiation effects* DNA Damage / physiology* Dose-Response Relationship, Radiation Electricity Electromagnetic Fields* Lipopolysaccharides / administration & dosage Mice Nitric Oxide / metabolism* Oxidative Stress / drug effects Oxidative Stress / physiology* Oxidative Stress / radiation effects RAW 264.7 Cells Radiation Dosage |
| IF | 2.368 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |